In the 13th February 2017, journal club meeting, the theoretical aspects of CompuSyn Software were discussed. The method, median effect equation, combination index, and the equations used in this statistical technique/method were described. It was mentioned that this method is used to evaluate the synergism, potentiation, and antagonism in combination therapies. It is used for a single drug and drug combinations pharmacodynamics with automated simulations, for dose-effect data analysis of enzymes, receptors, microorganisms, cells, animals and clinical trials. The measurement of IC50, IC95, ED50, LD10, LD50 were explained.