The topic of discussion on 14th April 2018 at Journal Club Meeting was ‘Exosomes: Novel bio-carriers for drug and gene therapy’. Exosomes may provide novel mechanisms and have potential to use as cell-free therapy. Exosomes can transport DNA, RNA, lipids or proteins executing both physiological as well as pathological role. They can enter circulation and can cross biological barriers and can be used as natural nanoparticles for drug or gene delivery. Stem cells also selectively employed for delivery of therapeutic genes or drug, and mesenchymal stem cells used as a carrier for drug delivery because of their ease in their isolation and expanded in vitro. In addition to stem cells, exosomes can be produced by tumor cell line, B cells, T cells, dendritic cells, macrophages, glial cells, and neurons. The exosome is a natural carrier of miRNA and can be used as gene delivery system. Mechanisms such as simple fusion through the cellular membrane, endocytosis or activation of target cells through the presence of specific surface ligands may be utilized for delivery of secreted exosomes to target cells. The yield of a pure exosome is the key problem for its clinical application. The use of exosomes as a carrier system for drug or gene is still in infancy, requiring more investigation for human application.